We have investigated the anti-inflammatory effects of Cinnamomum cassia constituents (cinnamic aldehyde, cinnamic alcohol,\r\ncinnamic acid, and coumarin) using lipopolysaccharide (LPS)-stimulated mouse macrophage (RAW264.7) and carrageenan\r\n(Carr)-induced mouse paw edema model. When RAW264.7 macrophages were treated with cinnamic aldehyde together with\r\nLPS, a significant concentration-dependent inhibition of nitric oxide (NO), tumor necrosis factor (TNF-a), and prostaglandin E2\r\n(PGE2) levels productions were detected.Western blotting revealed that cinnamic aldehyde blocked protein expression of inducible\r\nnitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear transcription factor kappa B (NF-?B), and I?Ba, significantly. In\r\nthe anti-inflammatory test, cinnamic aldehyde decreased the paw edema after Carr administration, and increased the activities\r\nof catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the paw tissue. We also demonstrated\r\ncinnamic aldehyde attenuated the malondialdehyde (MDA) level and myeloperoxidase (MPO) activity in the edema paw after\r\nCarr injection. Cinnamic aldehyde decreased the NO, TNF-a, and PGE2 levels on the serum level after Carr injection. Western\r\nblotting revealed that cinnamic aldehyde decreased Carr-induced iNOS, COX-2, and NF-?B expressions in the edema paw. These\r\nfindings demonstrated that cinnamic aldehyde has excellent anti-inflammatory activities and thus has great potential to be used as\r\na source for natural health products.
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